Figure 6.

Localization-independent loss of HDAC4 and HDAC3 interaction on calyculin A-treatment. Forty-eight hours after transfection with HDAC4-FLAG, TAg Jurkat cells were treated with staurosporine or calyculin A for 1.5 h. HDAC4-FLAG was immunopurified, and the sample was split into thirds. One-third of the immunopurified protein was prepared for Western blot analysis, one-third was incubated for 1 h with untreated TAg Jurkat lysate, and the remaining one-third of the sample was incubated with calyculin A-treated TAg Jurkat lysate for 1 h. These samples were analyzed for 14-3-3 and HDAC3 binding by Western blotting.