FIG. 5.

dys is expressed at sites of tracheal fusion. Whole-mount stage 15 and 16 embryos were stained with anti-Dys (green) and MAb 2A12 (red), which stains the tracheal lumen. Two segments are shown in panels A, B, and C; one segment is shown in panel D. Two Dys-positive cells can be seen at sites of tracheal fusion (panel A, panel B, and one segment of panel C), and one cell stained in each unfused branch at sites that will soon undergo fusion (panel D and one segment of panel C). Cells stained in all four types of tracheal fusion branches, including the dorsal trunk (DT) (A), the lateral trunk (LT) (B), the dorsal branch (DB) (C), and the ganglionic branch (GB) (D).