FIG. 7.

Dys and Tgo show nuclear colocalization. Embryos were double stained with α-Dys (green) and α-Tgo (red). Sagittal views of stage 14 (A to F, I, and J) and stage 15 (G and H) embryos are shown. Images on the left show double-labeled merges (fusion cells in C, E, G, and I are yellow), and the images on the right show only the α-Tgo red channel. Arrowheads indicate a Dys-positive cell for comparison between left and right images. (A and B) Leading-edge cells show nuclear accumulation of both Dys and Tgo. (C to J) Tracheal fusion cells show nuclear colocalization with Tgo and that Tgo nuclear levels are comparable to those of other tracheal cells. The tracheal branch type is shown to the left of the images.