FIG. 9.

dys-dsRNA-injected embryos show increased levels of Trh in fusion cells. Embryos from esg⁰⁵⁷³⁰ P[esg-lacZ]/CyO P[ftz-lacZ] parents were injected with dys-dsRNA and immunostained with α-β-Gal (esg-lacZ and ftz-lacZ) (blue), α-Dys (red), and α-Trh (green). Only heterozygous embryos were analyzed. Dorsal trunks at stages 14 and 17 are shown. Arrowheads indicate selected fusion cells. esg-lacZ is expressed at comparable levels in dys-dsRNA-injected embryos compared to wild-type or GFP-dsRNA (control)-injected embryos (see Fig. 6). Staining with α-Dys shows an absence of Dys protein. The Trh levels in fusion cells are significantly higher than in wild-type or control-injected embryos (see Fig. 6) and are comparable to the levels in adjacent tracheal cells. The two α-Trh rows are higher magnifications of the same embryo.