Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Aug;23(16):5540–5555. doi: 10.1128/MCB.23.16.5540-5555.2003

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, American Society for Microbiology

PMC Copyright notice

FIG. 7. — ΔNp73 cooperates with oncogenic Ras in promoting cellular transformation in vivo. (A) Characterization of MEFs for nude mouse assays used in Table 1. The percentage of ΔNp73 expressing cells as measured by GFP. FACS analysis of 129 MEFs cotransduced with ΔNp73 and K-Ras^V12 (top) and with ΔNp73 and N-Ras^D12 (bottom) immediately prior to subcutaneous injection into nude mice. (B) Photographs of nude mice 7 weeks after injections of ΔNp73+H-Ras^V12-infected 129 MEFs. (C) Corresponding sections of tumors generated by 129 MEFs cotransduced with the indicated retroviruses. Formalin-fixed and paraffin-embedded hematoxylin-eosin-stained sections show a typical sarcomatous morphology with tumor necrosis, anaplasia, and invasion into fat and muscle tissue. p53m indicates p53 R175H. Magnification, ×200. (D) ΔNp73 expression is maintained in fibrosarcomas. Immunohistochemical detection of ΔNp73 on a section from a ΔNp73+K-Ras^V12-generated tumor is shown. Detection was done by using biotin-streptavidin-horseradish peroxidase. Magnification, ×600.

FIG. 7. — ΔNp73 cooperates with oncogenic Ras in promoting cellular transformation in vivo. (A) Characterization of MEFs for nude mouse assays used in Table 1. The percentage of ΔNp73 expressing cells as measured by GFP. FACS analysis of 129 MEFs cotransduced with ΔNp73 and K-Ras^V12 (top) and with ΔNp73 and N-Ras^D12 (bottom) immediately prior to subcutaneous injection into nude mice. (B) Photographs of nude mice 7 weeks after injections of ΔNp73+H-Ras^V12-infected 129 MEFs. (C) Corresponding sections of tumors generated by 129 MEFs cotransduced with the indicated retroviruses. Formalin-fixed and paraffin-embedded hematoxylin-eosin-stained sections show a typical sarcomatous morphology with tumor necrosis, anaplasia, and invasion into fat and muscle tissue. p53m indicates p53 R175H. Magnification, ×200. (D) ΔNp73 expression is maintained in fibrosarcomas. Immunohistochemical detection of ΔNp73 on a section from a ΔNp73+K-Ras^V12-generated tumor is shown. Detection was done by using biotin-streptavidin-horseradish peroxidase. Magnification, ×600.