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. 2003 Aug;23(16):5825–5835. doi: 10.1128/MCB.23.16.5825-5835.2003

FIG. 3.

FIG. 3.

Extracellular domain YWTD-EGF repeat regions are responsible for LRP6 oligomer formation. (A) Schematic representation of additional LRP6 receptor deletion constructs (Flag or myc tagged) and of secreted extracellular subdomains (myc tagged). The transmembrane (TM) and cytoplasmic (C) domains of LRP6 were replaced by the TMC domains of FGFR2 to generate the LRP6-FGFR chimeric receptor. SP, signal peptide. (B) Comparison of signaling activities of different LRP6 deletion mutants in a TCF luciferase reporter assay. Analysis was performed following transfection of 293T cells with 0.25 μg of each LRP6 construct per well as described in the legend to Fig. 1C. (C and D) Comparison of receptor complex formation by different LRP6 mutants or extracellular subdomains. At 48 h following transfection of 293T cells with 2 μg of each LRP6 construct as indicated, immunoprecipitation (IP) with anti-Flag antibody and immunoblotting (IB) with anti-myc antibody were performed as described in the legend to Fig. 1D. All Flag-tagged constructs were expressed at comparable levels and immunoprecipitated efficiently with anti-Flag antibody (data not shown). Vec, vector; Ig, immunoglobulin.