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. 2003 Aug;23(16):5857–5866. doi: 10.1128/MCB.23.16.5857-5866.2003

FIG. 3.

FIG. 3.

The effect of increasing heme concentrations on the transformation of mutant HMCs to DCs. Approximately 20 μg of protein extracted from cells expressing wild-type Hap1 (lanes 19 to 24), H7-NDH (lanes 1 to 6), H7-N7 (lanes 7 to 12), H7-N (lanes 13 to 18), H7-DH (lanes 25 to 30), or H7-10A (lanes 31 to 36) was incubated with radiolabeled DNA in the presence of 0 (lanes 6, 12, 18, 24, 30, and 36), 0.1 (lanes 5, 11, 17, 23, 29, and 35), 0.2 (lanes 4, 10, 16, 22, 28, and 34), 0.5 (lanes 3, 9, 15, 21, 27, and 33), 1 (lanes 2, 8, 14, 20, 26, and 32), or 2 (lanes 1, 7, 13, 19, 25, and 31) μg of heme per ml. The reaction mixtures were loaded onto 3.5% nondenaturing polyacrylamide gels and electrophoresed, and the bands were visualized by using a PhosphorImager. These experiments were repeated at least twice.