TABLE 1.
Determination of telomere length by using Q-FISH in primary MEFsa
| Genotype and MEF group | No. of metaphases | Mean length (kb) ± SE |
No. of telomeres | No. of signal-free ends (%)b | ||
|---|---|---|---|---|---|---|
| p-arm | q-arm | Avg | ||||
| Rad54+/− DNA-PKcs+/− | ||||||
| E | 10 | 45.4 ± 0.51 | 54.3 ± 0.58 | 50.0 ± 0.40 | 1,600 | 7 (0.44) |
| F | 10 | 42.0 ± 0.45 | 49.4 ± 0.56 | 45.7 ± 0.37 | 1,652 | 3 (0.18) |
| A | 10 | 43.4 ± 0.47 | 54.1 ± 0.55 | 48.8 ± 0.40 | 1,588 | 4 (0.25) |
| A+E+F | 30 | 43.6 ± 0.28 | 52.5 ± 0.33 | 48.1 ± 0.23 | 4,840 | 14 (0.29 ± 0.08) |
| Rad54−/− DNA-PKcs+/− | ||||||
| B2 | 10 | 31.6 ± 0.23 | 38.6 ± 0.55 | 35.2 ± 0.33 | 1,588 | 0 (0.0) |
| C2 | 10 | 31.3 ± 0.32 | 36.3 ± 0.47 | 33.8 ± 0.29 | 1,688 | 8 (0.47) |
| B | 10 | 30.0 ± 0.36 | 41.3 ± 0.49 | 35.7 ± 0.34 | 1,580 | 3 (0.19) |
| E2 | 10 | 25.3 ± 0.34 | 37.0 ± 0.44 | 31.2 ± 0.31 | 1,644 | 15 (0.9) |
| B2+C2+B+E2 | 40 | 29.6 ± 0.18 | 38.3 ± 0.25 | 33.9 ± 0.16 | 6,500 | 33 (0.39 ± 0.19) |
| Rad54+/− DNA-PKcs−/− | ||||||
| C | 10 | 39.3 ± 0.47 | 47.3 ± 0.57 | 48.8 ± 0.39 | 1,544 | 4 (0.26) |
| D | 10 | 36.0 ± 0.41 | 41.4 ± 0.50 | 38.7 ± 0.33 | 1,676 | 9 (0.54) |
| C+D | 20 | 37.6 ± 0.31 | 43.8 ± 0.36 | 40.7 ± 0.25 | 3,220 | 13 (0.40 ± 0.14) |
| Rad54−/− DNA-PKcs−/− | ||||||
| A2 | 10 | 29.4 ± 0.38 | 35.0 ± 0.50 | 32.2 ± 0.32 | 1,536 | 8 (0.52) |
| D2 | 10 | 37.0 ± 0.38 | 44.4 ± 0.45 | 40.7 ± 0.31 | 1,732 | 7 (0.40) |
| A2+D2 | 20 | 33.4 ± 0.29 | 40.0 ± 0.36 | 36.7 ± 0.24 | 3,268 | 15 (0.46 ± 0.06) |
a
Letters refer to individual MEFs of the indicated genotype.
b
Average signal-free end percent values are shown with the standard error.