TABLE 2.

Spontaneously arising chromosomal aberrations in primary MEFs of the indicated genotypes as determined by Q-FISH

Parametera	Results obtained with MEF type:
	Rad54+/− DNA-PKcs+/−				Rad54+/− DNA-PKcs−/−			Rad54−/− DNA-PKcs+/−					Rad54−/− DNA-PKcs−/−
	A	E	F	Total	C	D	Total	B	E2	B2	C2	Total	A2	D2	Total
No. of metaphases	100	100	100	300	100	100	200	100	100	100	100	400	100	100	200
No. DIC*	0	0	0	0	0	0	0	0	0.01	0.02	0	0.0075	0.04	0.02	0.03
No. RL (+TTAGGG)*†	0	0	0	0	0.02	0	0.01	0	0	0	0	0	0.02	0.02	0.02
No. RL (−TTAGGG)*†	0	0	0	0	0	0.02	0.01	0	0	0	0	0	0	0	0
No. of R*	0	0	0	0	0	0.0	0	0	0	0	0.02	0.005	0.02	0.02	0.02
Totalb fusions (DIC+RL+R)*	0	0	0	0	0.02	0.02	0.02	0	0.01	0.02	0.02	0.0125	0.08	0.06	0.07
No. of chromosome breaks*	0.01	0.02	0	0.01	0	0.06	0.03	0.03	0.01	0.04	0	0.02	0.16	0.02	0.09
No. of chromatid breaks*	0.03	0.02	0.04	0.03	0.04	0.06	0.05	0.15	0.11	0.12	0.32	0.175	0.16	0.2	0.18
No. of gaps*	0.01	0.02	0.02	0.017	0	0.02	0.01	0.02	0.07	0.04	0.14	0.067	0.08	0.04	0.06
No. of fragments*	0.02	0.02	0	0.01	0	0	0	0.03	0.03	0.04	0.02	0.03	0.02	0.02	0.02
Double minutes*	0	0	0	0	0	0	0	0.04	0.05	0	0.08	0.0425	0.06	0.06	0.06

^aDIC, dicentric; RL, Robertsonian-like; R, rings. ∗, frequency of each aberration per metaphase. Values in boldface highlight differences between genotypes. Letters in subheadings refer to independent MEFs of the indicated genotype. †, the presence (+TTAGGG) or absence (−TTAGGG) of TTAGGG repeats at the fusion point is indicated in the case of RL fusions.

^bThat is, the total end-to-end chromosome fusions.