TABLE 3.
SKY analysis of end-to-end fusions in primary MEFs
| Parameter | Resultsa for MEF genotype group: |
||
|---|---|---|---|
| Rad54−/− DNA-PKcs+/− E2 | Rad54+/− DNA-PKcs−/− C | Rad54−/− DNA-PKcs−/− D2 | |
| No. of metaphases | 101 | 78 | 89 |
| No. of end-to-end fusionsb | 1 (9;13) | 3 (X;3), (1;4), (3;15) | 3 (X;4), (X;12), (1;4) |
| No. of chromosome breaks | 1 (14) | 0 | 0 |
| No. of chromatid breaks | 3 (14), (2), (9) | 2 (5), (ND) | 3 (4), (3), (ND) |
| No. of fragments | 1 (9) | 0 | 1 (centromeric [ND]) |
| No. of gaps | 1 (1) | 0 | 0 |
a
Values in parentheses refer to the chromosome(s) involved in the aberration. Letters in the column headings refer to the individual MEFs used for the analysis. ND, not determined.
b
All fusions were between nonhomologous chromosomes.