Skip to main content
. 2003 Aug;23(16):5606–5613. doi: 10.1128/MCB.23.16.5606-5613.2003

FIG. 3.

FIG. 3.

tTERT binding of telomerase RNA circularly permuted deletions (CPDs). (A) Wild-type RNA and CPDs tested for tTERT binding are indicated in the figure. Note the CPD14-40 RNA migrates significantly faster than wild-type and CPD RNAs due to the larger deletion of residues. For each set of binding reactions three different RNA concentrations were used (100, 10, and 1 mM from left to right). Except for the top panel (CPDs 1 to 3), wild-type and CPD14-40 telomerase RNAs were only tested at the 10 mM RNA concentration as positive and negative controls. (B) Summary of the tTERT binding to telomerase RNA CPDs compared to wild-type shown with the telomerase RNA secondary structure. The highlighted sequence indicates the RNA region deleted in each CPD. The CPD number is shown next to each highlighted RNA sequence from 5′ to 3′ along the RNA. The color indicates the relative level of tTERT binding. Red (++++) indicates wild-type binding. Orange (+++), purple (++), and light blue (+) indicate binding less than that of wild-type tTERT. Gray indicates no tTERT binding. Relevant telomerase RNA nucleotide positions are indicated on the figure.