FIG. 4.

Telomerase activity reconstituted with small telomerase RNA circularly permuted deletions (CPDs) within stem-loop IV. (A) Telomerase activity assays were reconstituted with wild-type or mutant telomerase RNAs as indicated in the figure. +1, +7, +13, and +19 on the right indicate telomerase extension products from the d(GGGGTT)₃ primer. L.C., loading control added to each reaction. (B) Summary of the reconstituted telomerase activity levels for the stem-loop IV CPDs. The residue numbers 128 and 144 indicate the RNA region targeted for smaller deletions. Each highlighted sequence indicates the RNA residues deleted in each smaller CPD. The color indicates the relative level of reconstituted activity; red indicates wild-type or near-wild-type activity, and light blue indicates low and less processive activity, similar to that of CPD 24. The arrow between residues 135 and 136 indicates relative level of reconstituted activity for a circular permutation that breaks the phosphodiester bond between residues 135 and 136 (135/136).