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. 2003 Aug;23(16):5606–5613. doi: 10.1128/MCB.23.16.5606-5613.2003

FIG. 5.

FIG. 5.

Stem-loop IV stimulates telomerase processivity. (A) Secondary structures of stem-loop IV nt 112 to 159 (left) and distal stem-loop IV nt 128 to 142 (right) are shown. Residue numbers are indicated on the structures. (B) Telomerase reconstituted with wild-type (wt) or t108 telomerase RNA was assayed for telomerase activity in the presence of 0, 6, or 60 pmol of stem-loop IV RNA (nt 112 to 159) or distal stem-loop IV RNA (nt 128 to 142). +1, +7, +13, and +19 on the right indicate telomerase extension products from the d(GGGGTT)3 primer. I.C., internal control for loading. (C) Telomerase reconstituted with wild-type or t108 telomerase RNA was assayed for telomerase activity alone or in the presence of 500 pmol of stem-loop IV, stem-loop IV (137-138 UU→AA) double point mutant or stem II nt 15 to 40. +1, +7, +13, and +19 on the right indicate telomerase extension products from the d(GGGGTT)3 primer. L.C., loading control for telomerase reactions.