FIG. 3.

Coimmunoprecipitation of human mtRNA polymerase with h-mtTFB1. A soluble HeLa cell mitochondrial extract was prepared as described in Materials and Methods. This extract was probed for the presence of h-mtRNA polymerase by Western analysis (lane 1). The same extract was immunodepleted with a polyclonal h-mtTFB1 antibody (lane 2) or the control preimmune serum (lane 3) and probed for the presence of the h-mtRNA polymerase polypeptide (∼139 kDa) in the corresponding immunoprecipitates (IP) by Western analysis. Positions of migration of molecular weight standards (in kilodaltons) are shown to the right of the figure.