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. 2003 Aug;23(16):5816–5824. doi: 10.1128/MCB.23.16.5816-5824.2003

FIG. 6.

FIG. 6.

Proposed model of the interactions between human mitochondrial transcription proteins during initiation of transcription at the human LSP. Human mtTFA (black) is shown bound to the LSP upstream of the site of transcription initiation (bent arrow). It is shown bending the DNA (parallel lines) at the promoter as part of its ability to activate transcription initiation as proposed by Fisher et al. (12). The C-terminal activation region of h-mtTFA (-C) is shown binding to h-mtTFB1 (or h-mtTFB2) based on the results of this study. In this model, h-mtTFB1 serves an adapter function to bridge an interaction between h-mtTFA and h-mtRNA polymerase at the promoter, which is demarcated by a specific h-mtTFA/DNA complex. The 1:1 complex shown between h-mtTFB1 (or h-mtTFB2) and h-mtRNA polymerase is drawn based on our ability to coimmunoprecipitate these proteins (Fig. 3), the report of Falkenberg et al. (9) that these proteins interact in vitro during copurification, and on the reports of Cliften et al. that sc-mtTFB physically interacts with mtRNA polymerase in S. cerevisiae (4, 5).