FIG. 7.

Reduced protein degradation impairs TNF-α-directed induction of NF-κB but does not reduce NF-κB activity during ER stress. PEK^+/+ and PEK^−/− MEF cells were treated with thapsigargin (Tg), TNF-α, and MG132 as indicated by the “+” or “−” symbols for the indicated number of hours or were subjected to no stress (0 h). Equal amounts of nuclear lysates prepared from MEF cells were analyzed for binding to the NF-κB probe by the EMSA. DNA complexed with p65/p50 or p50/p50 is indicated by arrows.