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p65 function is dispensable for increased expression of eIF2α kinase pathway genes in response to ER stress. p65 (RelA)+/+ (lanes 1 to 3) and p65−/− (lanes 4 to 6) MEF cells were exposed to thapsigargin (Tg) for 3 or 6 h or were subjected to no stress (0 h). Equal amounts of whole-cell lysates were separated by SDS-PAGE, and levels of p65, phosphorylated eIF2α, total eIF2α, ATF4, and Chop were measured by immunoblotting using antibodies specific to the indicated protein.
