FIG. 6.
Active Dnmt3a/3b isoforms rescue the capacity of late-passage 7aabb cells to form terotomas in nude mice. (A) The indicated ES cell lines were injected into nude mice subcutaneously on both sides (three to four mice for each cell line, 5 × 105 cells per site), and the mice were examined for teratomas after 4 weeks. A typical representation of the size of the teratomas derived from each cell line is shown. (B) Histological sections of teratomas derived from J1, early-passage (P10) 7aabb, and Dnmt3a, Dnmt3a2, and Dnmt3b1 stable clones showing the presence of multiple types of differentiated cells.