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. 2003 Aug;23(16):5768–5779. doi: 10.1128/MCB.23.16.5768-5779.2003

FIG. 2.

FIG. 2.

Ceg1p is concentrated at the promoters of PDR5, ASC1, and DBP2. The distribution of the TAP-tagged mRNA guanylyltransferase, Ceg1p, was compared in promoter and downstream regions of three genes (see gene diagrams). Input lanes 1, 4, and 7 show the starting extract from the TAP-tagged Ceg1p yeast strain, and Cl-4b lanes 2, 5, and 8 show no antibody control. High levels of Ceg1p were detected on promoter (pro) regions when the immunoprecipitation was performed with rabbit IgG-coated beads (lanes 3, 6, and 9), and the downstream regions of all three genes (+1.2kb and +4.2kb for PDR5, in and ex2 for DBP2, and ex2 for ASC1) showed a decrease in Ceg1p. The data shown here are representative of two (PDR5 and ASC1) and three (DBP2) independent ChIP experiments.