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. 2003 Aug;23(16):5768–5779. doi: 10.1128/MCB.23.16.5768-5779.2003

FIG. 3.

FIG. 3.

U1 snRNP levels remain constant along intronless genes. (A) ChIP analysis of three representative intronless genes—RPS3, ADH1, and PDR5—comparing promoter regions with downstream regions in the YKK19 strain. The data shown here are representative of two (RPS3 and PDR5) and three (ADH1) independent ChIP experiments. In these experiments, the ratio of Prp42p to Pol II signals at the downstream positions relative to promoters were 1.09 for RPS3, 0.88 for ADH1, and 0.93 and 0.62 for the two downstream positions in PDR5, which are ∼1.2 and ∼4.0 kb away from the promoter, respectively. (B) Activation of GAL1 by growth in galactose (lanes 5 to 8) causes the accumulation of Pol II but not Prp42p at the promoter. When grown in glucose (lanes 1 to 4), Pol II was strongly and Prp42p was weakly detected only on ADH1. Neither protein was detected at the telomere VI R. The data shown here are representative of four independent ChIP experiments.