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. 2003 Aug;23(16):5768–5779. doi: 10.1128/MCB.23.16.5768-5779.2003

FIG. 4.

FIG. 4.

Accumulation of the U1 snRNP on DBP2 depends on active transcription. Strain YKK20 contains a temperature-sensitive Pol II (rpb1-1) and an HA-tagged prp42p. When ChIP was performed on YKK20 grown at permissive temperature (24°C), the distribution of Pol II and Prp42p was determined as shown on DBP2 (lanes 1 to 4) and on ADH1, GAL1, and the telomere VI R (lanes 9 to 12). When the temperature was shifted to a nonpermissive level (37°C), Pol II no longer accumulated along DBP2 (lane 7) or ADH1 (lane 15), and Prp42p was not detected above control levels on DBP2 (lane 8) or ADH1 (lane 16). Neither protein was detected on GAL1 or the telomere VI R. The data shown here are representative of four independent ChIP experiments.