FIG. 5.

Deletion of the DBP2 intron abolishes cotranscriptional U1 snRNP accumulation. Prp42p was tagged at the C terminus with an HA tag in strain yIB37 that contains the DBP2 cDNA in place of the endogenous intron-containing ORF (2). As expected, no intron product was observed in the input (lane 2) compared to input from strain YKK19, which is wild type for DBP2 (lane 1). Equivalent amounts of template for mIgG control, anti-HA, or anti-Pol II were used for PCR (lanes 3 to 5). As a positive control, ChIP analysis is shown for ASC1 (lanes 6 to 10), which is wild type in this strain. The data shown here are representative of four independent ChIP experiments.