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. 2003 Aug;23(16):5768–5779. doi: 10.1128/MCB.23.16.5768-5779.2003

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FIG. 6. — Genome localization analysis of HA-Prp42 and Myc-Pol II. Cy3- and Cy5-labeled probes were synthesized from sheared genomic DNA or ChIP templates by linker-mediated PCR and hybridized with microarrays representing 6,229 yeast ORFs. Frequency histograms of ChIP scores [log₁₀(median intensity fluorochrome 1/median intensity fluorochrome 2)/√2̄] for representative experiments (Cy3-genomic/Cy5-genomic probes, top panel; Cy3-U1 snRNP/Cy5-genomic probes, middle panel; Cy3-Pol II/Cy5-genomic probes, bottom panel) show a normal distribution, with outlying second peaks in the U1 snRNP and Pol II experiments.