FIG. 7.

Reduced detection of the U1 snRNP on downstream regions of intron-containing genes with long second exons. PCR detection of the 5′ and 3′ ends of ECM33 and SAC6 genes in the YKK19 strain comparing levels of Pol II and Prp42p. A schematic diagram of each gene shows the layout of each ORF and the locations of 5′ and 3′ primer products. Levels of Pol II (lanes 4 and 8) were evenly distributed along the length of both genes. Prp42p levels (lanes 3 and 7) showed accumulation of the U1 snRNP on the 5′ end of both genes and decreasing levels in the 3′ regions. The data shown here are representative of four (ECM33) and three (SAC6) independent ChIP experiments.