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. 2003 Aug;23(16):5614–5624. doi: 10.1128/MCB.23.16.5614-5624.2003

FIG. 2.

FIG. 2.

Expression of ADAM15 (A, C, and E) and PECAM-1 (B, D, and F) in the endocardium and in the developing vasculature at E13.5. (A and B) Consecutive serial sections of the endocardial cushion of the heart of an E13.5 embryo stained with anti-ADAM15 antibody (A) or anti-PECAM-1 antibody (B). Strong expression of ADAM15 can be seen in endocardial cells, which here line the surface of the endocardial cushion. These cells are also positive for PECAM-1 staining. Scale bar, 100 μm. (C to F) Consecutive sections of the lower trunk and abdomen of an E13.5 embryo reveal an almost identical staining pattern for ADAM15 (C) as for PECAM-1 (D). Scale bar, 200 μm. At high magnification (the boxed areas in panels C and D), positive-staining reveals similar capillary structures in the sections stained with ADAM15 (E) and PECAM-1 (F). Scale bar, 100 μm. The staining of endothelial cells is specific for ADAM15, since it was not observed in mice lacking ADAM15 (see below, Fig. 4). However, the staining of blood cells by the ADAM15 antibody is nonspecific, since this staining is not abolished in adam15−/− mice (see Fig. 4D). (G) Western blot analysis confirmed that ADAM15 is expressed in HUVECs.