FIG. 7.

Tumor growth is retarded in adam15^−/− mice. Wild-type (WT) and adam15^−/− (−/−) mice were injected subcutaneously with 10⁶ B16F0 cells. (A) Comparison of the final weight of tumors grown in mixed-genetic-background (129/SvJ and C57BL/6) wild-type and adam15^−/− mice. The results represent the combination of five independent experiments. (B) Comparison of the final weight of the tumors grown in wild-type and adam15^−/− mice in a predominantly C57BL/6 background (six backcrosses). The data represent the combination of three independent experiments. To account for possible differences in the number of injected cells and their growth potential between individual experiments, as well as different durations of the experiments, the average tumor weight in wild-type animals in each experiment was used as a reference to calculate the weight of each tumor in percentages of this average. The bars in panels A and B represent the average value for wild-type and adam15^−/− mice. (C) Sections of the tumors grown in wild-type and adam15^−/− mice stained with hematoxylin and eosin or immunostained with anti-PECAM-1 antibody. No apparent difference in histological morphology or vascular density was observed between the two genotypes. Scale bar, 100 μm.