Table 2.
Translocation and elongation times
| Protein | Elongation rate*†, aa/sec | Elongation time‡, sec | Tshift§, sec | Translocated chain length¶, aa |
|---|---|---|---|---|
| BR:H6 | 1.8 ± 0.4 | 140 ± 30 | ||
| N-terminal domain | 67 | 130 | ||
| I4C:H6 | 1.9 ± 0.4 | 130 ± 30 | ||
| T5C:H6 | 1.8 ± 0.4 | 140 ± 30 | ||
| BC loop | 25 | 210 | ||
| E74C:H6 | 1.9 ± 0.4 | 130 ± 30 | ||
| Q75C:H6 | 1.8 ± 0.3 | 140 ± 20 | ||
| FG loop | −28‖ | >251 | ||
| A196C:H6 | 1.7 ± 0.2 | 150 ± 20 | ||
| I198C:H6 | 1.6 ± 0.2 | 160 ± 20 |
*
Values reported are the average of seven experiments with one SD.
†
Elongation rates were calculated by fitting elongation time courses (see Materials and Methods).
‡
Elongation times were calculated by dividing the number of amino acids in the mature protein by the elongation rate.
§
Tshift values were estimated as described in Results.
¶
The translocated chain length was calculated by (elongation time − Tshift) × 1.8, where 1.8 is the average elongation rate.
‖
A negative value indicates that translocation occurs after elongation.