FIG. 8.

Inactivation of Rac1 during apoptosis. JLP-119 cells were induced to undergo apoptosis by treatment with VP-16 (8.5 μM) for the indicated times. Cells were lysed by sonication in the presence of GST-tagged PBD and precipitated by mixing with glutathione-Sepharose beads. The presence of Rac1 was detected by immunoblotting with anti-Rac1 (C-11). Top panel, active Rac1 present in the GST-PBD precipitates; bottom panel, Rac1 remaining in the supernatant fractions after mixing with GST-PBD beads. The percentage of apoptotic cells at each time point is indicated above each lane. The results are representative of three independent experiments.