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. 2003 Aug;23(16):5716–5725. doi: 10.1128/MCB.23.16.5716-5725.2003

FIG. 9.

FIG. 9.

Expression of caspase 3-resistant Rac1 mutants in JLP-119 and BL-41 cells inhibits drug-induced apoptosis. Stable transfectants of JLP-119 cells (A and B) or BL-41 cells (C and D) were treated with 8.5 μM or 170 μM VP-16, respectively, for the indicated times. The cell lines expressed either empty pCDNA3.1(+) vector (open circles), HA-tagged dominant-negative Rac1N17 (solid circles), constitutively active Rac1V12 (open triangles), or caspaseresistant Rac1D11E (solid triangles). Apoptosis was assayed by nuclear morphology following Hoechst dye and propidium iodide staining and fluorescence microscopy (A and C). Expression of HA-tagged recombinant Rac1 protein was followed by Western blot immunoassay with an anti-HA antibody (B and D).