FIG. 7.

Increased flk1⁺ cells in embryoid bodies stably expressing HoxB5. (A) Single-cell suspensions were prepared from wild-type or stably transfected embryoid bodies at day 4, and the expression of flk1 was analyzed by flow cytometry with phycoerythrin-conjugated rat anti-mouse flk1 antibody. The y axis represents the relative cell number; the x axis represents the fluorescence intensity. Gray lines indicate negative control, light lines represent results obtained with the addition of specific antibody, and dark lines represent the flk1⁺ population. The percentages of cells that fall within the indicated gate are noted. Values of <2% represent background staining. A significantly increased number of cells expressing the mouse flk1 gene were observed in the HoxB5-transfected embryoid bodies. (B) Comparison of flk1⁺ cells in HoxB5- and HoxB6-overexpressing embryoid bodies.