TABLE 2.
Yeast strains and plasmids used in this study
| Name | Description or relevant properties | Source or reference |
|---|---|---|
| Strains | ||
| LS1-9 | LS1-4-26BMATαrec102Δ1::ura3-52ade5 CYH2smet13d trp5d leu1-12CAN1sura3-52 ade2-1 | This study |
| LS1-4-74C MATα rec102Δ1::ura3-52ADE5cyh2rmet13c trp5c leu1-ccan1rura3-52 ade2-1 | ||
| LS2-8 | LS2-3-23DMATαlys2-1 tyr1-1 his7-1ADE5 met13c cyh2rtrp5c leu1-cCAN1sura3-13ade2-1 | 42 |
| LS2-3-37A MATα lys2-1 tyr1-1 his7-2ade5 met13dCYH2strp5d leu 1-12can1rura3-13 ade2-1 | ||
| LS7-1 | LS2-3-4B1-1MATαrec104Δ-1rec102Δ2::LYS2lys2-1 tyr1-1 his7-1 ADE5 met13d cyh2rtrp5d leu1-12can1rura3-13ade2-1 | This study |
| LS2-3-20D1-1 MATα rec104Δ-1rec102Δ2::LYS2lys2-1 tyr1-1 his7-2 ade5 met13cCYH2strp5c leu1-cCAN1sura3-13ade2-1 | ||
| JK9-1 | Isogenic to LS2-8; homozygous rec102Δ2::LYS2 | This study |
| JK9-4 | Isogenic to LS2-8; homozygous rec102-18 | This study |
| LS5-1 | Isogenic to LS2-8; hemizygous rec104-8/rec104Δ-1 | 42 |
| K65-3D | HOMATαlys2-1 tyr1-1 his7-2can1rura3-13ade5 met13-d trp5-2 leu1-12ade2 | 18 |
| HOMATα lys2-1 tyr1-1 his7-2can1rura3-13ade5 met13-d trp5-2 leu1-12 ade2 | ||
| JK5-1-5D | Same as K65-3D except rec102-Δ2::LYS2/rec102-Δ2::LYS2 | This study |
| JK12-4 | Same as JK5-1-5D except spo11Δ::kanr/spo11Δ::kanr | This study |
| LS8-1-8B | Same as JK12-4 except rec104Δ1::URA/rec104Δ1::URA3 | This study |
| LS8-1-3B | Same as K65-3D except spo11::kanr/spo11::kanrrec104Δ1::URA3/rec104Δ1::URA3 | This study |
| Plasmids | ||
| pCM212 | REC102 in pRS316 | 12 |
| pEG(KG) | GST fusion protein expression vector; 2μm origin URA3 | 32 |
| pESC-URA | FLAG and MYC expression vector | Stratagene |
| pJK21 | ClaI site in polylinker region of pCM212 disrupted | This study |
| pJK22 | REC102 in pRS426 | This study |
| pJK27 | rec102Δ2::LYS2 in pRS426 | This study |
| pJK46 | rec102-18 allele in pRS316 | This study |
| pJK55 | rec102-18 allele in pRS306 | This study |
| pJK61 | REC102 in modified pRS316 with NsiI site disrupted | This study |
| pJK63 | SPO11 from pJK72 cloned in pRS316 | This study |
| pJK64 | SPO11 from pJK72 cloned in pRS426 | This study |
| pJK72 | Original clone of high-copy-number suppressor of rec102-18 | This study |
| pJK73 | Another original clone of high-copy-number suppressor of rec102-18 | This study |
| pJK74 | 3-HA tag fused with REC102 in pRS316 | This study |
| pJK75 | 3-HA tag fused with REC102 in pRS426 | This study |
| pJK81 | 2μm origin TYR1 | This study |
| pJK108 | REC102-3-HA tag in pJK81 | This study |
| pJK113 | GST-SPO11 fusion construct in pEG(KG) | This study |
| pLS37 | REC104 cloned into pESC-URA | This study |
| pLS741 | pJK74 with REC102-48 mutation | This study |
| pMH1 | REC104 cloned into pJK81 | This study |
| pMPY-3xHA | Yeast 3-HA epitope tagging vector | 42 |
| pRS306 | Integration vector; URA3 | 45 |
| pRS316 | CEN6 ARSH4 URA3 | 45 |
| pRS426 | 2μm origin URA3 | 11 |
| YDp-K | Integration vector; LYS2 | 4 |