TABLE 3.
Mutant rec102 alleles and their locations
| Allele no. | Phenotype | Altered amino acid(s)a |
|---|---|---|
| rec102-5 | Tsb | R4Q, E29D, F62V, E95K, Q166L |
| rec102-6 | Ts | D56N, K65E, F112S |
| rec102-7 | Ts | S48G, R67K, F122L |
| rec102-8 | Ts | F122S, S129T |
| rec102-10 | Ts | N109L |
| rec102-14 | Ts | Q36H, S118F |
| rec102-17 | Ts | E110G, Q119L |
| rec102-18 | Ts | Q175L |
| rec102-19 | Ts | Y111C |
| rec102-21 | Partial | D167G |
| rec102-27 | Null | I24F, R180G |
| rec102-29 | Null | V117M, W138R, A149S |
| rec102-39 | Null | S114F |
| rec102-45 | Null | E11G, H72R, S113F |
a
PCR mutagenesis of REC102 was carried out as described in Materials and Methods, and mutants were isolated as described in the text. Mutations in bold occur in amino acids conserved among S. cerevisiae, S. paradoxus, and S. pastorianus (21).
b
Ts, temperature sensitivity.