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. 2003 Aug;23(16):5726–5737. doi: 10.1128/MCB.23.16.5726-5737.2003

FIG. 2.

FIG. 2.

NO increases PI3 kinase activity. (A) HSVEC were stimulated by GSNO (300 μΜ), and increases in phosphatidylinositol-3,4,5-trisphosphate (pip3) production were measured with and without wortmannin (Wort, 25 nm). (B) HSVEC were stimulated z increasing concentrations of GSNO (3 to 300 μΜ) or SNAP (100 μΜ) for 1 h, and cell lysates were subjected to the PI3 kinase activity assay. (C) HSVEC protein lysates after immunoprecipitating (IP) by an antibody to phosphotyrosine were treated with GSNO (300 μΜ) or with dithiothreitol (DTT, 1 mM) for 30 min before the PI3 kinase activity assay. Lysates from HSVEC stimulated by GSNO (300 μΜ) were used as a positive control. (D) PI3 kinase activity in HSVEC stimulated or not 8-bromo-cGMP (300 μΜ) for 1 h. (E) PI3 kinase activity in endothelial cells stimulated by GSNO in the presence or absence of the soluble guanylyl inhibitor ODQ (1 μΜ) for 1 h. Untreated cells were used as a negative control.