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. 2003 Aug;23(16):5726–5737. doi: 10.1128/MCB.23.16.5726-5737.2003

FIG. 3.

FIG. 3.

NO promotes endothelial cell migration via PI3 kinase and cGMP-dependent protein kinase. (A) HAEC migration through the membrane filter in response to 10 μΜ and 100 μΜ SNAP was analyzed in a Boyden chamber. Original magnification, 200×. (B) Cell migration was measured in BAEC (percent of control) treated with increasing concentrations of SNAP (1 to 300 μΜ). (C) Migration was measured in BAEC treated with SNAP (100 μΜ) in the presence or absence of the PI3 kinase inhibitors wortmannin (Wort, 25 nm) and LY294002 (LY, 10 μΜ) or the soluble guanylyl cyclase inhibitor ODQ (1 μΜ). (D) Cell migration was measured in BAEC (percent of control) treated with increasing concentrations of 8-bromo-cGMP (cGMP, 10 to 1000 μΜ). (E) Migration was measured in BAEC treated with 8-bromo-cGMP (300 μΜ) in the presence or absence of wortmannin (Wort, 25 nm) or LY294002 (LY, 10 μΜ). Asterisks indicate a statistically significant difference (P < 0.05) between the treated cells and untreated cells (control, Ctrl). The migration data presented are shown as mean ± standard error of the mean. Each experiment was performed in quadruplicate.