FIG. 3.
Fluorescence microscopy of B. subtilis mutant cells. (A to C) (Left panels) Fluorescence of SMC-YFP. (Right panels) DNA stained by DAPI. (A) Strain JM33 (smc-yfp ypuI::tet). (B) Strain JM31 (smc-yfp scpA::tet). (C) Strain JM32 (smc-yfp scpB::tet). Arrowheads in panels A to C indicate anucleate cells containing SMC-YFP. (D) Strain JM8 (scpA-yfp). (E) Strain JM29 (scpA-yfp smc-cfp). (F) Strain JM9 (scpB-yfp). (G) Strain JM30 (scpB-yfp smc-cfp). (H) Strain JM35 (phyperspac-smc-yfp) with 1 mM IPTG; arrowheads indicate anucleate cells. (I) Strain JM36 (phyperspac-smc scpB-yfp) with 1 mM IPTG. Thin white lines indicate septa between cells. Thick white bars, 2 μm.
