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Ethidium-bromide stained 7% native polyacrylamide mobility shift assay. Linear DNA (1.5 pmol; 500 ng; 500 bp) was run in the absence (minus) or presence of SMC (20 pmol); SMC, ScpA, and ScpB (20 pmol each; compl); ScpA (20 pmol); ScpB (20 pmol); head domain (hd; 20 and 40 pmol, from left to right); or hinge (20 and 40 pmol).
