FIG. 12.

Ectopic c-Rel expression in human MCF-10F untransformed mammary epithelial cells induces levels of cyclin D1, and p52 and p50 expression. Cells were cotransfected with pSVSport-c-Rel (c-Rel) and pGKpuro plasmid expression vectors, and a mixed population of cells was selected with puromycin. WCEs were prepared from transfected cells and parental untransfected cells (Control), and samples (10 μg) were subjected to immunoblot analysis for c-Rel, RelA, p52/p100, p50/p105, cyclin D1, and β-actin levels using two identical blots that were successively reprobed with the different antibodies. To better visualize the p105 versus p50 band, a longer exposure was used.