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. 2003 Aug;23(16):5738–5754. doi: 10.1128/MCB.23.16.5738-5754.2003

FIG. 8.

FIG. 8.

MMTV-c-rel tumors overexpress c-myc mRNA. (A) Mammary glands. Total RNA was prepared from mammary glands and samples (15 μg) subjected to Northern analysis for c-myc mRNA expression. As a control, the gel was stained with ethidium bromide, shown below. The relative values of c-myc signal intensity normalized to levels of 28S rRNA are given relative to the WT (2) virgin sample. (B) Mammary tumors. Total RNA was extracted from the indicated mammary tumors (T) and grossly normal mammary glands (N) of age-related multiparous line 14 and 16 MMTV-c-rel mice. In addition, RNA was isolated from mammary glands of a nulliparous transgenic line 16 mouse (16 Virgin). RNA samples (20 μg) were subjected to Northern analysis for c-myc mRNA expression. As controls for RNA integrity and equal loading, the gel was stained with ethidium bromide, and RNA samples subjected to RT-PCR analysis for β-actin mRNA levels (Fig. 3). The values of c-myc signal intensity normalized to 28S rRNA levels relative to the line 16 virgin sample are given below.