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. 2003 Aug;23(16):5526–5539. doi: 10.1128/MCB.23.16.5526-5539.2003

FIG. 6.

FIG. 6.

Pak5 phosphorylates BAD independently of PKA and prevents Akt dephosphorylation on serine 473. (A) Stable cell lines were transfected with pEBG-BAD and after 24 h were pretreated 30 min with a specific myristoylated protein kinase A inhibitor (1 μM) before CA (10 μM) was added for 16 h. GST-BAD was then retrieved from the lysates and an anti-phospho-Ser-112 BAD antibody was used for the Western blot to assess BAD phosphorylation status. (B) Stable cell lines were treated for 16 h with CA (10 μM) or C2C (50 μM). After lysis, Western blotting was performed using an anti-phospho-Ser 473 specific antibody.