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. 2003 Aug;23(16):5849–5856. doi: 10.1128/MCB.23.16.5849-5856.2003

FIG. 2.

FIG. 2.

TRAF2 transactivates the LKLF promoter in TRAF2−/− cells. (A) TRAF2−/− mouse fibroblast cells were cotransfected with 0.5 μg of LKLF(−2047)-Luc, 0.2 μg of pRSV-LacZ, and 1 μg of an empty vector plasmid or the expression vector of TRAF2, TRAF2(87-501), TRAF2(272-501), or TRAF2DZ. Twenty-four hours after transfection, the cells were collected, and luciferase activity was detected and normalized to β-galactosidase activity. Data shown are the average values from three independent experiments. (B) TRAF2−/− cells were cotransfected with 0.5 μg of LKLF(−2047)-Luc, 0.2 μg of pRSV-LacZ, and 1 μg of an empty vector plasmid or the expression vector of TRAF2 or p65. Fourteen hours after transfection, one plate of cells transfected with the empty vector was treated with TNF (30 ng/ml) for 10 h. Cells were then collected for the luciferase assay, which was performed as described above for panel A. (C) As described above for panels A and B, TRAF2−/− cells were cotransfected with LKLF(12047)-Luc, pRSV-LacZ, and an empty vector or the expression vector of TRAF1, -2, -3, -4, -5, or -6. Luciferase activity of each transfection was determined as described above for panels A and B.