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TRAF2 localizes in the cytoplasm of the cells. HeLa cells were treated with LMB (10 μg/ml) for 15 h or were not treated (−). Nuclear (N) and cytoplasmic (C) fractions of the cells were isolated and Western blotted for TRAF2 or c-Myc, which was used as a control for nuclear proteins. The positions of molecular mass markers (in kilodaltons) and of TRAF2 and c-Myc proteins are indicated to the right of the blots.
