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. 2003 Aug;23(16):5849–5856. doi: 10.1128/MCB.23.16.5849-5856.2003

FIG. 4.

FIG. 4.

Transactivation of the LKLF promoter by TRAF2 requires p38 MAP kinase. (A) TRAF2−/− mouse fibroblast cells were cotransfected with 0.5 μg of LKLF(−2047)-Luc, 0.2 μg of pRSV-LacZ, and 1 μg of an empty vector or the expression vector of TRAF2. As indicated, some cells were treated (+) with SB203580 (SB) (20 μM), SP600125 (SP) (20 μM), or U0126 (5 μM). Cells were collected 24 h after transfection, and the luciferase assay was performed as described in the legend to Fig. 2. The expression of TRAF2 in TRAF2-transfected cells was detected with an anti-FLAG antibody. (B) TRAF2−/− mouse fibroblast cells were cotransfected with 0.5 μg of LKLF(−2047)-Luc, 0.2 μg of pRSV-LacZ, and 1 μg of the indicated plasmids. The luciferase assay was performed as described in the legend to Fig. 2. The expression of TRAF2 was detected with an anti-FLAG antibody. (C) The protein levels of LKLF in WT and p38−/− mouse fibroblast cells were detected by Western blotting. β-Actin was used as a protein input control.