FIG. 5.

LKLF protected TRAF2^−/− cells against TNF-induced apoptosis. (A) Cell extracts from WT, TRAF2^−/−, and LKLF stably transfected TRAF2^−/− mouse fibroblast cells (LKLF clones 3 and 7) were used to detect protein expression of LKLF and TRAF2. (B) LKLF stably transfected TRAF2^−/− cells, TRAF2^−/− LKLF clones 3 and 7 (LKLF #3 and LKLF #7, respectively), and pHygromycin (Hygro)-transfected TRAF2^−/− cells were treated with TNF (20 ng/ml) with the indicated concentration of CHX for 8 h, and dead cells were determined by trypan blue staining as described previously (21). The percentages shown are the average values from three independent experiments. (C) The cells described above for panel B were treated with CHX (200 ng/ml) and the indicated concentration of TNF for 8 h, and dead cells were determined by trypan blue staining.