FIG. 6.

The expression of LKLF in TRAF2^−/− cells has no effect on TNF-induced IKK and JNK activation. (A) WT, TRAF2^−/−, or TRAF2^−/− LKLF clone 3 cells were treated with mouse TNF (20 ng/ml) for 10 min. Equal cell extracts were used for in vitro kinase assay to measure IKK activity and were Western blotted for the protein levels of IKKβ. (B) WT, TRAF2^−/−, and TRAF2^−/− LKLF clone 3 cells were treated with mouse TNF (20 ng/ml) for 15 min. JNK1 protein expression or activity from each sample was measured by Western blotting or JNK kinase assay. The positions of molecular mass markers (in kilodaltons) are indicated to the left of the blots.