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. 2003 Aug;23(16):5849–5856. doi: 10.1128/MCB.23.16.5849-5856.2003

FIG. 7.

FIG. 7.

Impact of IGF-IR expression on TNF-induced cell death in TRAF2−/− cells. (A) Equal amounts of cell extracts from WT, TRAF2−/−, and TRAF2-transfected TRAF2−/− cells were Western blotted (WB) for the protein levels of IGF-IRβ or β-actin. (B) Equal amounts of cell extracts from WT, TRAF2−/−, and TRAF2−/− LKLF clone 3 cells were Western blotted for the protein levels of IGF-IRβ or β-actin. (C) Equal amounts of cell extracts from TRAF2−/− and TRAF2−/− mouse fibroblast cells stably transfected with IGF-IR or pHygromycin were Western blotted for the protein level of IGF-IRβ. The positions of molecular mass markers (in kilodaltons) are indicated to the left of the blots. (D) TRAF2−/− and TRAF2−/− mouse fibroblast cells stably transfected with IGF-IR or pHygromycin (Hygro) were treated with TNF (20 μg/ml) and CHX (200 ng/ml) for 8 h. Dead cells were determined by trypan blue staining. The results shown are average values for three independent experiments.