FIG. 6.

Graphical representation of decay of CYC1, CYH2 and ACT1 mRNAs at 25°C (A, C, and E) and at 37°C (B, D, and F) from thiolutin treated cells of NUP116 (•), nup116-Δ (○), nup116-Δ cbc1-Δ (▾), nup116-Δ rrp6-Δ (▿), and nup116-Δ cbc1-Δ rrp6-Δ (▪). The decay was determined by Northern blot analysis of the RNA extracted from the strains mentioned above treated with thiolutin from 0 to 50 min. The result from one typical experiment from each strain at different temperatures are presented as the percentage of mRNA remaining versus time of incubation of thiolutin.