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Poly(A) tail analysis of mRNA from strains carrying the nup116-Δ mutation. Poly(A) tails were analyzed by 3′ end labeling of 1 μg of total RNA with [32P]CP and RNA ligase, followed by hydrolysis with RNase A and RNase T1, electrophoretic separation on a 16% acrylamide-8 M urea gel, and storage phosphorimager analysis. Lane M indicates pBR322 MspI-cut length markers.
