TABLE 3.

Half-lives of ACT1, CYH2, and CYC1 mRNAs in various strains^a

Pertinent genotype	Half-life (min) of:
	ACT1		CYH2		CYC1
	25°C	37°C	25°C	37°C	25°C	37°C
NUP116	52 ± 4.5	32 ± 1.5	50 ± 3.5	26 ± 2.0	50 ± 3.5	31 ± 2.5
nup116-Δ	48 ± 3.25	18 ± 1.5	55 ± 4.0	11 ± 1.0	45 ± 2.0	18 ± 1.5
nup116-Δ cbc1-Δ	54 ± 4.25	60 ± 2.0	57 ± 3.75	37 ± 3.0	65 ± 3.0	61 ± 1.0

^aThe half-lives were determined with total RNA from thiolutin-treated cells as described in Materials and Methods. The values of multiple determinations fell within 15% of the mean. Where applicable, the mean values are presented (determined from three independent experiments) to nearest whole number. The number after the mean value represents the range of three independent experiments.