TABLE 4.

Comparison of half-lives of ACT1, CYH2, and CYC1 mRNAs in different mutant strains under the restrictive condition of 37°C after temperature shift^a

Pertinent genotype	Half-life (min) of:
	ACT1	CYH2	CYC1
NUP116	32 ± 1.5	26 ± 2.0	31 ± 2.5
nup116-Δ	18 ± 1.5	11 ± 1.0	18 ± 1.5
nup116-Δ cbc1-Δ	60 ± 2.0	37 ± 3.0	61 ± 1.0
nup116-Δ rrp6-Δ	56 ± 6.5	28 ± 3.0	52 ± 5.5
nup116-Δ rail-Δ	38 ± 2.5	30 ± 2.5	38 ± 3.0
nup116-Δ upf1-Δ	16 ± 1.5	10 ± 1.0	24 ± 1.0
nup116-Δ cbc1-Δ rrp6-Δ	44 ± 3.0	26 ± 2.0	48 ± 3.5
HPR1	34 ± 3.5	NDb	ND
hpr1-Δ	22 ± 1.5	ND	ND

^aThe half-lives were determined with total RNA from thiolutin-treated cells as described in Materials and Methods. The values of multiple determinations fell within 15% of the mean. Where applicable, the mean values are presented (determined from three independent experiments) to nearest whole number. The number after the mean value represents the range of three independent experiments.

^bND, not determined.