FIG. 7.
NFull promotes accumulation of SuH RNA more strongly than NΔCterm. (a) NFull promotes accumulation of RNA of Enhancer of split m3, a target of SuH- and Nintra-dependent N signaling. The promotion is strong in the presence of Dl (compare lanes 2 and 4 with lane 6 in the upper panel). RNA was extracted from cells immediately after mixing of the different cells (0 min) or after 45 min. (b) NFull also promotes accumulation of SuH RNA. c. NΔCterm promotes expression of Enhancer of split m3 and su(H) RNA only weakly, if at all. The blots were reprobed with rp 49 probe to reveal relative levels of total RNA in the lanes. Forty micrograms of total RNA was loaded in all lanes. m3 and rp 49 probes were generated using the primers described by Mishra-Gorur et al. (30a). SuH probe was generated using the full coding sequence.